老年人桡骨远端骨折影像学参数测量与临床疗效的关系

目的 :通过分析老年人桡骨远端骨折患者经过保守治疗后各影像学参数和临床疗效之间的关系,从而提出老年人桡骨骨折行手法复位中最重要的影像学参数。方法:回顾性分析自2012年5月至2015年5月行保守治疗的60岁以上的桡骨远端骨折患者45例,男17例,女28例;年龄60~89岁,平均64.49岁;随访测量影像学参数以并评价临床疗效。影像学参数是在标准腕关节X线片上测量掌倾角、桡骨高度、尺骨变异、尺偏角等;腕关节临床疗效通过Dienst评分标准来评价;多因素线性回归法分析各影像参数与临床疗效的关系。结果 :45例患者均获得随访,时间12~24个月,平均17.6个月;所有桡骨远端骨折获得愈合。末次随访根据Dienst评价:优27例,良10例,可6例,差2例。影像学参数指标中桡骨高、掌倾角以及尺骨变异和自测腕关节功能有明显相关性。当桡骨高8.12 mm、掌倾角-1.64°、尺骨变异4.05 mm时,Dienst功能结果优良率较高。结论 :桡骨高、掌倾角及尺骨变异等影像学参数与老年桡骨远端骨折的临床疗效恢复有相关性,其中最重要的参数为桡骨高度,提醒术者对于老年人桡骨骨折行手法复位时复位前后尤为注意该参数。     

数字建模分析倒退旋转法在腰椎椎体单侧穿刺椎体后凸成形术的适用性

目的:利用Mimics软件建立腰椎的3D模型,使用模拟穿刺来观察倒退旋转法在腰椎椎体单侧穿刺椎体后凸成形术的适用性及进针参数。方法:选取24例全腰椎螺旋CT扫描初次胸椎骨质疏松性骨折、腰椎无异常骨质破坏的影像学资料,其中男女各12例,应用Mimics软件对原始DICOM文件进行3D建模,分离出各节段椎体,直接导入3-matic软件后约束其椎体后壁进行标准化测量后以椎弓根中点作一垂直于中切面及椎体后壁的素描图,在素描图上进行模拟穿刺,记录穿刺针可活动范围的角度参数及距离参数,记录穿刺针在最大外倾角时落在椎体中切线前、中、后区的穿越点,并进行比较分析。结果:所有数据在左右两侧差异均无统计学意义(P0.05),不同节段数据在两性差异有统计学意义(P0.05),男女性L_1-L_5最大外倾角均逐渐增大,男性从(33.41±1.31)°到(56.53±4.71)°,女性从(28.58±2.55)°到(53.86±2.68)°,所有穿越点未落在前区,其中女性26.67%落在中区,男性3.33%落在中区,A、B、C分区在性别上差异有统计学意义(P0.05)。结论:倒退旋转法理论上均能满足椎体压缩性骨折对穿刺点的要求,其中男性及下腰椎更加适用倒退旋转法。最大外倾角的测定对倒退旋转法有指导性意义。     

股骨颈骨折内固定失败Ⅱ期行髋关节置换的早期疗效

目的:研究青壮年股骨颈骨折患者内固定失败后Ⅱ期行髋关节置换的早期临床疗效。方法:2008年6月至2010年6月,对24例股骨颈骨折内固定术后股骨头坏死患者行全髋关节置换术,其中男12例,女12例;年龄18～58岁,平均42.9岁。术后行X线检查,并根据Harris评分标准评价临床疗效。结果:23例获得随访,平均随访时间34.4个月(25～48个月),1例术后并发髋关节脱位,未发现髋关节感染、松动或假体周围骨折等并发症。Harris评分为90.9±4.3,优18例,良4例,可1例。结论:Ⅱ期全髋关节置换治疗股骨颈骨折内固定术后并发股骨头坏死,术中手术难度增加,但可以获得良好的早期疗效。     

A pregnancy with discordant fetal and placental chromosome 18 aneuploidies revealed by invasive and noninvasive prenatal diagnosis

This study investigated a pregnancy where the fetus was diagnosed with monosomy 18p by invasive amniocentesis and karyotyping. Additional noninvasive prenatal diagnosis, which detects fetal chromosome abnormalities in the circulating cell-free plasma DNA originating from the placenta revealed a related 18p monosomy/18q trisomy, suggesting confined placental mosaicism. Based on recent observations of chromosomal instability in the early preimplantation embryo, this study speculates on the possible embryonic origin(s) of these related but discordant chromosome 18 aneuploidies in the placental and fetal tissues. The findings highlight the potential for both false-positive and -negative noninvasive prenatal diagnosis results in pregnancies where there is either confined placental mosaicism or placental mosaicism.The study investigated a pregnancy involving a fetus with a chromosome disease syndrome called monosomy 18p where part of the short arm of chromosome 18 was missing in the fetal tissues. Using non-invasive prenatal diagnosis which detects fetal chromosome abnormalities in the circulating cell free plasma DNA originating from the placenta, we also detected monosomy 18p as well a related chromosome 18 abnormality involving duplication of the long arm of chromosome 18. This suggested confined placental mosaicism where the constitution of the chromosomes are different between fetal and placental tissues. We speculated that these related chromosome 18 abnormalities arose during preimplantation embryo development, leading to the formation of different chromosome abnormalities observed in the placental and fetal tissues of this pregnancy. Our findings highlight the potential for both false positive and negative non-invasive prenatal diagnosis test results in pregnancies where there is confined placental mosaicism.     

系统性硬化病患者足部创面修复1例

<正>患者,女,43岁,因系统性硬化病20年、双足皮肤缺损6月余入院。患者20年前无明显诱因出现雷诺现象,双手遇冷后会变白变紫,保暖后可好转,后逐渐出现四肢指(趾)端皮肤肿胀、僵硬及萎缩,并向近心端蔓延,进食时有梗咽感并伴有四肢散在红斑,后逐渐出现口干、眼干及负重时胸闷气促感,在外院     

Preparation,characterization and application of anti-human OX40 ligand (OX40L) monoclonal antibodies and establishment of a sandwich ELISA for autoimmune diseases detection

OX40L (CD252, TNFSF4), a type II transmembrane protein which like other tumor necrosis factor ligands, involved in the costimulation and differentiation of T cells, functions as a positive signal in immune response. To investigate the biological function of soluble OX40L (sOX40L), three functional anti-OX40L monoclonal antibodies (mAbs) 3D2, 3F7 and 2H3 were obtained by hybridoma technology. Besides, specificity of the mAbs was further demonstrated by ELISA, Western blot and Immunofluorescence experiments. We also developed a novel enzyme-linked immunosorbent assay (ELISA) based on two anti-human OX40L antibodies 3D2 and 3F7 with different epitopes. Using the ELISA system, we found that sOX40L in the sera of healthy donors increases in an age-dependent manner and that enhanced sOX40L expression in some autoimmune diseases especially in rheumatoid arthritis (RA) patients, suggesting the potential diagnostic significance of sOX40L in the autoimmune diseases. Together, these data demonstrate that the existence of circulating sOX40L in human sera might play an important role in immunoregulation.     

Enhanced osseointegration of grit-blasted,NaOH-treated and electrochemically hydroxyapatite-coated Ti–6Al–4V implants in rabbits

Osseointegration, in terms of the bone apposition ratio (BAR) and the new bone area (NBA), was measured by backscattered electron imaging. The results were compared for four implant types: grit-blasted and NaOH-treated Ti–6Al–4 V (Uncoated-NaOH), electrodeposited with hydroxyapatite without alkali treatment (ED-HAp), electrodeposited with hydroxyapatite after alkali treatment (NaOH-ED-HAp), and plasma sprayed with hydroxyapatite (PS-HAp). No heat treatment was done after soaking in NaOH. The implants were press fitted into the intramedullary canal of mature New Zealand white rabbits and analyzed, both at the diaphyseal and at the metaphyseal zones, either 1 week or 12 weeks after surgery. NaOH-ED-HAp already exhibited a higher BAR value than the ED-HAp at 1 week, and was as good as the commercial PS-HAp at 12 weeks. The NBA value for NaOH-ED-HAp at 12 weeks was the highest. The higher content of octacalcium phosphate in NaOH-ED-HAp, as evident from the X-ray photoelectron spectroscopy analysis of the oxygen shake-up peaks, and the associated increase in the solubility of this coating in vivo are considered responsible for the enhanced osseointegration. Taking into account also the reduced occurrence of delamination and the inherent advantages of the electrodeposition process, electrodeposition of HAp following soaking in NaOH may become an attractive alternative for the traditional plasma-sprayed process for coating of orthopedic and dental implants.     

后内外不同入路治疗内侧延伸型后踝骨折疗效比较

目的：探讨经后内侧和后外侧两种术式治疗内侧延伸型后踝骨折的临床疗效。方法：自2008年1月至2011年1月,采用空心拉力螺钉治疗内侧延伸型后踝骨折患者25例。15例采用经后内侧切口入路拉力螺钉内固定（后内侧切口组）,其中男9例,女6例;年龄21-67岁,平均（48.1±1.3）岁。按Denis-Weber AO骨折分型：A型5例,B型6例,C型4例。10例采用经后外侧切口拉力螺钉内固定（后外侧切口组）,其中男6例,女4例;年龄23-64岁,平均（46.9±1.5）岁。Denis-Weber AO骨折分型：A型3例,B型5例,C型2例。记录并比较两组患者的手术时间、术中出血量、切口长度、X线暴露次数及并发症发生情况,术后采用Baird-Jackson评定标准对两组患者的功能评价。结果：25例患者均获随访,时间12-49个月,平均20.6个月。两组患者手术时间、术中出血量、X线暴露次数、并发症情况比较差异有统计学意义（P〈0.05）;两组患者疗效比较差异无统计学意义（P〉0.05）。结论：后内侧入路治疗内侧延伸型后踝骨折,可以在直视下显露和固定骨折块,具有手术耗时短、X线暴露次数少、术中出血量少的优点,是值得选择的手术入路方式。     

Do post-COVID-19 symptoms exist? A longitudinal study of COVID-19 sequelae in Wenzhou,China

ObjectiveWe conducted a cohort of tracing discharge patients of COVID-19.Materials and methodsWe used the Mann–Whitney U test, χ² test, or Fisher's exact test to compare differences between age groups and gender groups where appropriate.ResultsOur study provides insights into the nature and severity of medical conditions specific to survivors of COVID-19.ConclusionsIt also highlights the potential mental health issues resulting from infectious disease outbreaks within communities.